| Impact of Buprenorphine on Post-operative Clinical Sign and Corticosterone Levels |
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The use of laboratory animals in experiment often requires surgical operations. Post-operative condition often associated with pain and stress. Pre emptive analgesia and post operative treatment with buprenorphine can reduce post operative pain and stress and prevent pain become chronic.
The aim of the present study was to test the hypothesis that pre
operative treatment of buprenorphine reduces stress and suppresses the
pre-operative serum corticosterone levels in Fischer 344 rats. A total
of 14 adult male Fischer 344 (F344/Sca) rats permanently catheterized
in artery carotis communis were used. During 94 hour post-operatively
the blood sample was collected at specific time point using
Accusampler®, an automated blood sampling. Pre and post operative body
weight and water intake were registered daily. Buprenorphine 1.0 mg/kg
significantly suppressed serum corticosterone levels immediately after
rats regain consciousness. Rats in buprenorphine 1.0 mg/kg group were
protected from post operative body weight loss and reduced water intake
compare to control, nutella, and buprenorphine 0.4 mg/kg group.
Quantification of fecal corticosteroid metabolites failed to detect
stress in present study. Buprenorphine is also effective as pre emptive
analgesia in F344 strain. However the dose is higher than what has been
reported in Sprague Dawley strain. |
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